Western blot analysis of Cas9 in extracts of 293T cells and 293T transfected with CAS9 protein. Samples were incubated with Cas9 Polyclonal antibody (Product # PA5-90171) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.